Mutation of a putative AMPK phosphorylation site abolishes the repressor activity but not the nuclear targeting of the fungal glucose regulator CRE1.
Curr Genet
; 37(5): 328-32, 2000 May.
Article
in En
| MEDLINE
| ID: mdl-10853770
ABSTRACT
In filamentous ascomycetes, glucose repression is mediated by CRE1, a zinc-finger protein related to Miglp from yeast. Five putative AMPK phosphorylation motifs identified in the glucose repressor from the phytopathogenic fungus Sclerotinia sclerotiorum were mutated in a GFPCRE1 translational fusion. Complementation experiments in Aspergillus nidulans and fluorescence microscopy analyses showed that mutation of one site (Ser266) abolishes the repressor activity of the fusion protein but not its nuclear targeting, suggesting that an AMPK protein kinase may be involved in the function of the fungal glucose repressor.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Repressor Proteins
/
Fungal Proteins
/
Protein Serine-Threonine Kinases
/
DNA-Binding Proteins
/
Glucose
/
Multienzyme Complexes
/
Mutation
Type of study:
Prognostic_studies
Language:
En
Journal:
Curr Genet
Year:
2000
Document type:
Article
Affiliation country:
Alemania