Regeneration of halved embryonic lower first mouse molars: correlation with the distribution pattern of non dividing IDE cells, the putative organizers of morphogenetic units, the cusps.
Int J Dev Biol
; 44(3): 289-95, 2000 Apr.
Article
in En
| MEDLINE
| ID: mdl-10853825
ABSTRACT
Recently we demonstrated that non-cycling, cap-stage, mouse molar inner dental epithelial (IDE) cells corresponding to the primary enamel knot (EK) area underwent a coordinated temporo-spatial patterning leading to their patchy irregular segregation at the tips of the forming cusps. These non-cycling cells were suggested to perhaps represent the organizers of the morphogenetic units (OMU), the cusps. The present study has analyzed the regenerative capacity of halved cap-stage first lower mouse molars through three dimensional (3D) reconstructions. Partial regeneration of the anterior half and possible complete regeneration of the posterior half were documented. Using BrdU (5-bromo-2'-deoxyuridine) labeling and 3D reconstructions of the IDE, we have correlated the patterns of cusp regeneration with the distribution of BrdU negative IDE cells. These data support a morphogenetic role for the non-cycling IDE cells.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Cuspid
/
Molar
Limits:
Animals
Language:
En
Journal:
Int J Dev Biol
Journal subject:
BIOLOGIA
/
EMBRIOLOGIA
Year:
2000
Document type:
Article
Affiliation country:
Francia