Cellular distribution of isozymes of protein kinase C in septal olfactory epithelium of mice.
Neurosci Lett
; 288(2): 143-6, 2000 Jul 14.
Article
in En
| MEDLINE
| ID: mdl-10876081
ABSTRACT
To determine the presence of protein kinase C (PKC) isozymes in the septal olfactory epithelium of mice (mSOE), western blotting and immunohistochemistry were performed using antibodies against PKC isozymes. With the exception of PKC-betaI, all of the PKC isozymes were detected in the whole lysate of septal tissue layer and apparent molecular weights for each isoform were found. PKC-alpha, PKC-gamma and PKC-epsilon were detected in the olfactory glandular cells of the lamina propria, and PKC-betaI and PKC-betaII were located in the microvillar cells. Neither novel PKC nor atypical PKC was detected in olfactory glandular cells or microvillar cells, except for PKC-epsilon. PKC-lambda was localized in the mucous layer of the mSOE. Meanwhile, PKC-delta and PKC-xi were distributed in the receptor cells in the mSOE. These data demonstrate the isoform-specific expression of PKC in mSOE and suggest a role for the novel and atypical types of PKC in olfactory transduction.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Protein Kinase C
/
Olfactory Mucosa
Limits:
Animals
Language:
En
Journal:
Neurosci Lett
Year:
2000
Document type:
Article
Affiliation country:
Corea del Sur