Adenoviral-mediated transfer of the TNF-related apoptosis-inducing ligand/Apo-2 ligand gene induces tumor cell apoptosis.
J Immunol
; 165(5): 2886-94, 2000 Sep 01.
Article
in En
| MEDLINE
| ID: mdl-10946322
ABSTRACT
TNF-related apoptosis-inducing ligand (TRAIL) is a member of the TNF superfamily of cytokines that induces apoptosis in a variety of cancer cells. The results presented in this study demonstrate that introduction of the human TRAIL gene into TRAIL-sensitive tumor cells using an adenoviral vector leads to the rapid production and expression of TRAIL protein, and subsequent death of the tumor cells. Tumor cell death was mediated by an apoptotic mechanism, as evidenced by the activation of caspase-8, cleavage of poly(ADP-ribose) polymerase, binding of annexin V, and inhibition by caspase inhibitor zVAD-fmk. These results define a novel method of using TRAIL as an antitumor therapeutic, and suggest the potential use for an adenovirus-encoding TRAIL as a method of gene therapy for numerous cancer types in vivo.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Membrane Glycoproteins
/
Tumor Cells, Cultured
/
Adenoviruses, Human
/
Tumor Necrosis Factor-alpha
/
Apoptosis
Limits:
Humans
/
Male
Language:
En
Journal:
J Immunol
Year:
2000
Document type:
Article
Affiliation country:
Estados Unidos