Determination of amobarbital and secobarbital in plasma samples using micellar liquid chromatography.
Biomed Chromatogr
; 14(5): 287-92, 2000 Aug.
Article
in En
| MEDLINE
| ID: mdl-10960826
ABSTRACT
A new liquid chromatographic procedure for the determination of amobarbital and secobarbital in plasma samples is proposed. The method uses a Spherisorb octadecylsilane ODS-2 C(18) analytical column, a guard column of similar characteristics and 0.04 M CTAB solution buffered at pH 7.5 containing 3% 1-propanol as micellar mobile phase. The UV detection was carried out at 250 nm. Butabarbital was used as internal standard. Plasma samples preparation only required adequate dilution with the mobile phase before injection into the chromatographic system. The limits of detection were 0.2 and 0.4 mg/L for amobarbital and secobarbital, respectively. The proposed method allows the determination of amobarbital and secobarbital in plasma at therapeutic levels.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Secobarbital
/
Chromatography, Liquid
/
Amobarbital
/
Hypnotics and Sedatives
Type of study:
Diagnostic_studies
Limits:
Humans
Language:
En
Journal:
Biomed Chromatogr
Year:
2000
Document type:
Article
Affiliation country:
España