The growth suppressing gas1 product is a GPI-linked protein.
FEBS Lett
; 481(2): 152-8, 2000 Sep 15.
Article
in En
| MEDLINE
| ID: mdl-10996315
ABSTRACT
Growth arrest specific (gas) 1 gene product is expressed in non-transformed fibroblasts in response to stimuli driving cells into Go phase. Gas1 has been demonstrated to inhibit cell proliferation when over-expressed in proliferating fibroblasts. This activity depends on a function of the p53 protein independent of its transactivating ability. To better define the pathway leading from Gas1, which is located on the plasma membrane, to p53, we have undertaken a detailed characterization of its topology. We demonstrate that the protein undergoes cotranslational modifications in the endoplasmic reticulum, consisting of signal peptide cleavage, N-linked glycosylation and glycosyl-phosphatidylinositol anchor addition. Immunoelectron microscopy shows that, in its mature form, Gas1 is randomly distributed over the outer leaflet of the plasma membrane and that upon antibody-induced clustering it relocalizes to caveolae.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Membrane Glycoproteins
/
Cell Membrane
/
Glycosylphosphatidylinositols
/
Saccharomyces cerevisiae Proteins
Limits:
Animals
/
Humans
Language:
En
Journal:
FEBS Lett
Year:
2000
Document type:
Article
Affiliation country:
Italia