Molecular cloning of ring finger protein 21 (RNF21)/interferon-responsive finger protein (ifp1), which possesses two RING-B box-coiled coil domains in tandem.
Genomics
; 69(1): 143-9, 2000 Oct 01.
Article
in En
| MEDLINE
| ID: mdl-11013086
ABSTRACT
We have cloned the full length of a novel cDNA, named ring finger protein 21 (RNF21), composed of the RING finger-B box-coiled coil (RBCC) domain and the B30.2 domain, which are characteristic of the RBCC-B30.2 family. As a structural feature, the RNF21 cDNA possessed at least three kinds of isoforms, due to alternative splicing, consisting of the long form with the RBCC-RBCC-B30.2 domain, the medium form with the RBCC-B30.2 domain, and the short form with only the RBCC domain. Moreover, respective transcripts corresponding to the three isoforms were detected in various human organs by reverse transcription-PCR and Northern blot analyses. Interestingly, the medium form of the RNF21 mRNA expressed most predominantly was dramatically up-regulated within 8-16 h by interferon stimulation of HeLa cells. These findings suggest that RNF21 is a downstream gene that may mediate interferon's biological action.
Search on Google
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Carrier Proteins
/
Zinc Fingers
Limits:
Animals
/
Female
/
Humans
/
Male
Language:
En
Journal:
Genomics
Journal subject:
GENETICA
Year:
2000
Document type:
Article
Affiliation country:
Japón