Insulin-dependent phosphorylation of a 70-kDa protein in light microsomes from rat adipocytes.
Biochem Biophys Res Commun
; 276(3): 1302-5, 2000 Oct 05.
Article
in En
| MEDLINE
| ID: mdl-11027626
ABSTRACT
In order to discover possibly novel insulin receptor substrates and/or downstream targets in the insulin signaling pathway, we established a cell-free system for this purpose using purified insulin receptor and subcellular fractions from rat adipocytes as a sourse of cellular substrates. Under these conditions, we have found a 70-kDa protein (pp70) in fat cells that is tyrosine-phosphorylated by the activated insulin receptor. Using sucrose velocity gradient sedimentation we also show that pp70 cofractionate a particulate fraction containing IRS-1 but not with GLUT-4 vesicle-enriched fractions. Our results suggest that pp70 may be an endogenous substrate for the insulin receptor tyrosine kinase.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Phosphoproteins
/
Adipocytes
/
Phosphotyrosine
/
Insulin
/
Microsomes
/
Muscle Proteins
Limits:
Animals
/
Humans
/
Male
Language:
En
Journal:
Biochem Biophys Res Commun
Year:
2000
Document type:
Article
Affiliation country:
Estados Unidos