Inhibition of human peripheral blood neutrophil respiratory burst by alcohol-based venipuncture site disinfection.
Clin Diagn Lab Immunol
; 7(6): 980-2, 2000 Nov.
Article
in En
| MEDLINE
| ID: mdl-11063510
ABSTRACT
Ethanol inhibits the respiratory burst of neutrophils. Therefore, the effects of alcohol-based skin disinfection on oxygen metabolism in neutrophils were tested using 70% ethanol or an ethanol-isopropanol-n-propanol mixture. Neutrophil respiratory burst activity as assessed fluorometrically by oxidation of 2', 7'-dichlorofluorescein diacetate increased at 10 min after disinfection with 70% ethanol compared to the activity at 30 s. The increase was significant for triggering oxidative burst with formyl-peptide but not with phorbol myristate acetate.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Respiratory Burst
/
Disinfectants
/
Alcohols
/
Neutrophils
Limits:
Humans
Language:
En
Journal:
Clin Diagn Lab Immunol
Journal subject:
ALERGIA E IMUNOLOGIA
/
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Year:
2000
Document type:
Article
Affiliation country:
Italia