MCI-186: further histochemical and biochemical evidence of neuroprotection.
Life Sci
; 67(19): 2387-92, 2000 Sep 29.
Article
in En
| MEDLINE
| ID: mdl-11065185
ABSTRACT
The bioactivity of 3-methyl-1-phenyl-pyrazolin-5-one (MCI-186) was examined based on histochemical changes in drastic global ischemic rat brains. Rats with mean arterial blood pressure reduction were subjected to 60 min cerebral ischemia/80 min reperfusion. Infusion of MCI-186 at 3.0 mg/Kg reduced brain infarction from 21 +/- 4% (saline control, n= 15) to 11 +/- 3% (n=16, p<0.05). By comparison, infusion of up to 20 mg/Kg propyl galalate (PG)--a well documented antioxidant--produced an infarct percentage of 14 +/- 5% (n=8), close to the saline control. Biochemically, the neuroprotective effect of MCI-186 was demonstrated by diminishing the release of creatine kinase (CK) in serum from 3363 +/- 608 U/L (n=14) in saline control to 1989 +/- 293 U/L (n= 15) in MCI group (p<0.05), while PG did not lower the activity of CK significantly. MCI-186 behaves as a free radical scavenger by suppressing the formation of superoxide anion in xanthine oxidase (XO)-hypoxanthine (HP) system (p<0.05). Our data supported our contention that MCI-186 has potent anti-stroke effect with antioxidant activities.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Brain
/
Reperfusion Injury
/
Antipyrine
/
Free Radical Scavengers
/
Neuroprotective Agents
/
Brain Infarction
Type of study:
Etiology_studies
Limits:
Animals
Language:
En
Journal:
Life Sci
Year:
2000
Document type:
Article
Affiliation country:
Canadá