Determination of enantiomeric purity of hyoscyamine from scopolia extract using HPLC-CD system without chiral separation.
Enantiomer
; 5(3-4): 369-75, 2000.
Article
in En
| MEDLINE
| ID: mdl-11126877
ABSTRACT
Enantiomeric ratio of hyoscyamine from Scopolia extract was determined by chiral HPLC-CD analysis. It was found that circular dichroism (CD) detection allowed the analysis of the sample without any special pretreatment whereas UV detection required an ammonia-ether extraction. To obtain a shorter analysis time for the determination, reversed-phase HPLC-CD analysis was applied by using a g-factor calibration curve (EE% vs. CD/UV). The analysis time was shortened from 35 to 18 min. EE% values obtained were consistent with those by chiral HPLC analysis.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Plants, Medicinal
/
Atropine
/
Plant Extracts
/
Chromatography, High Pressure Liquid
Language:
En
Journal:
Enantiomer
Journal subject:
BIOQUIMICA
Year:
2000
Document type:
Article
Affiliation country:
Japón