N-linked carbohydrate on human leukocyte antigen-C and recognition by natural killer cell inhibitory receptors.
Hum Immunol
; 61(12): 1202-18, 2000 Dec.
Article
in En
| MEDLINE
| ID: mdl-11163076
ABSTRACT
The possible role of carbohydrate in the interaction of HLA-C with a human inhibitory natural Killer cell Immunoglobulin-like Receptor with two Ig domains, KIR2DL1, was investigated. Transfectants of 721.221 (a class I MHC-negative human B cell line) expressing only HLA-Cw4 or -Cw6 or their respective non-glycosylated mutants (N86Q, S88A) were made. The binding of a KIR2DL1-Ig fusion protein to the non-glycosylated mutant HLA-Cw4- or -Cw6-expressing cells was markedly decreased compared to the wild type-expressing cells. The ability to induce an inhibitory signal in the NK tumor line YTS transfected with KIR2DL1 was also impaired in the nonglycosylated mutant expressing cells. Furthermore, in a second functional assay, mutant HLA-Cw4 and -Cw6 molecules had impaired ability to induce signal transduction in BW cells expressing a KIR2DL1-CD3 zeta chain chimeric protein. Thus, the deletion of the N-linked glycosylation signal in HLA-Cw4 and -Cw6 greatly reduced recognition by KIR2DL1. Alternative interpretations of the data are discussed.
Search on Google
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Membrane Glycoproteins
/
Killer Cells, Natural
/
Receptors, Immunologic
/
HLA-C Antigens
/
Antigens, CD
/
Lectins, C-Type
/
Carbohydrate Metabolism
Language:
En
Journal:
Hum Immunol
Year:
2000
Document type:
Article
Affiliation country:
Estados Unidos