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Monoliths as stationary phases for separation of proteins and polynucleotides and enzymatic conversion.
Josic, D; Buchacher, A; Jungbauer, A.
Affiliation
  • Josic D; Octapharma Pharmazeutika Produktions Ges.m.b.H., Vienna, Austria.
J Chromatogr B Biomed Sci Appl ; 752(2): 191-205, 2001 Mar 10.
Article in En | MEDLINE | ID: mdl-11270861
ABSTRACT
Monoliths are considered as a novel generation of stationary phases. They were applied for capillary electrochromatography and liquid chromatography exploiting every action principle such as ion-exchange, affinity recognition, reversed-phase, and hydrophobic interaction. The fast separation was explained by convective transport of the solutes through the bed. The contribution of this mode of transport is similarly explained as done for the beds packed with particles with gigapores. For monolithic beds, the concept of an ultrashort bed was frequently used. This mode of operation allows very short separation time. In many cases a gradient elution is necessary to achieve separation. Examples of applications for protein and polynucleotide separation performed on monoliths are given. Enzymatic conversion was described showing the examples of several immobilzed enzymes.
Subject(s)
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Collection: 01-internacional Database: MEDLINE Main subject: Polynucleotides / Proteins / Enzymes Language: En Journal: J Chromatogr B Biomed Sci Appl Journal subject: QUIMICA CLINICA Year: 2001 Document type: Article Affiliation country: Austria
Search on Google
Collection: 01-internacional Database: MEDLINE Main subject: Polynucleotides / Proteins / Enzymes Language: En Journal: J Chromatogr B Biomed Sci Appl Journal subject: QUIMICA CLINICA Year: 2001 Document type: Article Affiliation country: Austria