Identification of methyl halide-utilizing genes in the methyl bromide-utilizing bacterial strain IMB-1 suggests a high degree of conservation of methyl halide-specific genes in gram-negative bacteria.
Appl Environ Microbiol
; 67(4): 1959-63, 2001 Apr.
Article
in En
| MEDLINE
| ID: mdl-11282657
ABSTRACT
Strain IMB-1, an aerobic methylotrophic member of the alpha subgroup of the Proteobacteria, can grow with methyl bromide as a sole carbon and energy source. A single cmu gene cluster was identified in IMB-1 that contained six open reading frames cmuC, cmuA, orf146, paaE, hutI, and partial metF. CmuA from IMB-1 has high sequence homology to the methyltransferase CmuA from Methylobacterium chloromethanicum and Hyphomicrobium chloromethanicum and contains a C-terminal corrinoid-binding motif and an N-terminal methyltransferase motif. However, cmuB, identified in M. chloromethanicum and H. chloromethanicum, was not detected in IMB-1.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Bacterial Proteins
/
Genes, Bacterial
/
Gram-Negative Bacteria
/
Hydrocarbons, Brominated
/
Methyltransferases
Type of study:
Diagnostic_studies
Language:
En
Journal:
Appl Environ Microbiol
Year:
2001
Document type:
Article
Affiliation country:
Reino Unido