Kinetics study of the biotransformation of an oligonucleotide prodrug in cells extract by matrix-assisted laser desorption-ionization time-of-flight mass spectrometry.
J Chromatogr B Biomed Sci Appl
; 753(1): 123-30, 2001 Mar 25.
Article
in En
| MEDLINE
| ID: mdl-11302437
ABSTRACT
The fate of a dodecathymidine prodrug in cell extract was monitored by MALDI-TOF MS. This technique allows a facile identification and a relative quantification of metabolites produced. We showed that the relative peak intensities were similar to the relative metabolite proportions that permitted the determination of their half-lives. We found a good fit between the calculated kinetics curves and the experimental points. The oligonucleotide prodrug was fully metabolized to yield the dodecathymidine phosphorothioate likely through a carboxyesterase mediated mechanism.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Oligonucleotides
/
Prodrugs
/
Cell Extracts
Language:
En
Journal:
J Chromatogr B Biomed Sci Appl
Journal subject:
QUIMICA CLINICA
Year:
2001
Document type:
Article
Affiliation country:
Francia