Cerebellar alterations induced by chronic hypoxia: an immunohistochemical study using a chick embryonic model.
Brain Res
; 901(1-2): 271-6, 2001 May 18.
Article
in En
| MEDLINE
| ID: mdl-11368977
ABSTRACT
A model of fetal aerogenic hypoxia was developed in which fertilized chicken eggs were half-painted with melted wax and incubated under normal conditions. The cerebellum of the hypoxic chick embryos at a later stage of development (E18-20) was analyzed immunochemically. Hypoxic insult resulted in considerable neurocytological deficits of the Purkinje cells and altered glial fibrillary acid protein (GFAP) immunoreactivity in the fetal cerebellum. Purkinje cells in the hypoxic embryos were marked by small cell size, poorly developed dendrites, low cell density, deletion and ectopia. On the other hand, enhanced GFAP immunoreactivity was found in astrocytes and Bergmann glia of the hypoxic embryos. Our results indicate that chronic hypoxia in the chick fetus can cause severe disorders of neuronal development as well as glial activation. We suggest that our hypoxic model of chick embryos could be an accessible animal model for further elucidating fetal hypoxia.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Cerebellum
/
Hypoxia, Brain
/
Fetal Hypoxia
/
Nervous System Malformations
Limits:
Animals
Language:
En
Journal:
Brain Res
Year:
2001
Document type:
Article
Affiliation country:
Corea del Sur