Protective effect of lipophilic antioxidants on phorbol-induced acute lung injury in rats.
Crit Care Med
; 29(4): 819-24, 2001 Apr.
Article
in En
| MEDLINE
| ID: mdl-11373476
ABSTRACT
OBJECTIVE:
To determine whether the lipophilic antioxidant U-74389G can ameliorate the acute lung injury induced by phorbol myristate acetate (PMA) in our isolated lung model in rats, and to compare its activity with the intracellular enzymes superoxide dismutase (SOD) or catalase.DESIGN:
Randomized, controlled study.SETTING:
Animal-care facility procedure room.SUBJECTS:
Forty-two adult male Sprague-Dawley rats each weighing 250-350 g.INTERVENTIONS:
Typical acute lung injury was induced successfully by PMA during 60 mins of observation. PMA (2 microg/kg) elicited a significant increase in microvascular permeability (measured by using the capillary filtration coefficient Kfc), lung weight gain, the lung weight/body weight ratio, pulmonary arterial pressure, and the protein concentration of the bronchoalveolar lavage fluid. MEASUREMENTS AND MAINRESULTS:
Pretreatment with 1 mg of U-74389G significantly attenuated the acute lung injury induced by PMA, all parameters having decreased significantly (p <.001). The protective effect of U-74389G was dose dependent, but SOD (6,000 U/kg) or catalase (50,000 U/kg) exhibited no protective effect.CONCLUSIONS:
U-74389G significantly ameliorates acute lung injury induced by PMA in rats.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Pregnatrienes
/
Tetradecanoylphorbol Acetate
/
Lung Diseases
/
Antioxidants
Limits:
Animals
Language:
En
Journal:
Crit Care Med
Year:
2001
Document type:
Article
Affiliation country:
China