Ultrasound-mediated disruption of cell membranes. I. Quantification of molecular uptake and cell viability.
J Acoust Soc Am
; 110(1): 588-96, 2001 Jul.
Article
in En
| MEDLINE
| ID: mdl-11508983
ABSTRACT
Ultrasound-mediated drug delivery is a nonchemical, nonviral, and noninvasive method for targeted transport of drugs and genes into cells. Molecules can be delivered into cells when ultrasound disrupts the cell membrane by a mechanism believed to involve cavitation. This study examined molecular uptake and cell viability in cell suspensions (DU145 prostate cancer and aortic smooth muscle cells) exposed to varying peak negative acoustic pressures (0.6-3.0 MPa), exposure times (120-2000 ms), and pulse lengths (0.02-60 ms) in the presence of Optison (1.7% v/v) contrast agent. With increasing pressure and exposure time, molecular uptake of a marker compound, a calcein, increased and approached equilibrium with the extra cellular solution, while cell viability decreased. Varying pulse length produced no significant effect. All viability and molecular uptake measurements collected over the broad range of ultrasound conditions studied correlated with acoustic energy exposure. This suggests that acoustic energy exposure may be predictive of ultrasound's nonthermal bioeffects.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Ultrasonics
/
Tumor Cells, Cultured
/
Cell Membrane
/
Cell Survival
/
Muscle, Smooth, Vascular
Type of study:
Diagnostic_studies
Limits:
Humans
/
Male
Language:
En
Journal:
J Acoust Soc Am
Year:
2001
Document type:
Article
Affiliation country:
Estados Unidos