A study of the Autographa californica multiple nucleopolyhedrovirus ODV envelope protein p74 using a GFP tag.
J Gen Virol
; 82(Pt 9): 2279-2287, 2001 Sep.
Article
in En
| MEDLINE
| ID: mdl-11514740
ABSTRACT
The Autographa californica multiple nucleopolyhedrovirus (AcMNPV) protein p74 is associated with the occlusion-derived virus (ODV) envelope. p74 is essential for oral infectivity of ODV and has been proposed to play a role in midgut attachment and/or fusion. In this study, p74 protein was expressed in-frame with green fluorescent protein (GFP) to create a p74-GFP chimera. The C-terminal GFP portion of the chimera facilitated visualization of the trafficking of p74 in baculovirus-infected Spodoptera frugiperda (Sf-9) cells. p74-GFP chimeric proteins localized in the intranuclear ring zone of the nucleus and were found to co-precipitate with the microvesicle fraction of cell lysates. A series of truncations of p74 was expressed as p74-GFP chimeras in recombinant baculoviruses. When C-terminal region S580-F645 was deleted from p74, p74-GFP chimera localization became non-specific and chimeras became soluble. p74 region S580-F645 directed GFP to the intranuclear ring zone in a similar pattern to full-length p74. The hydrophobic C terminus of p74 plays a role in protein localization and possibly in transmembrane anchoring and insertion.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Viral Envelope Proteins
/
Nucleopolyhedroviruses
/
Luminescent Proteins
Limits:
Animals
Language:
En
Journal:
J Gen Virol
Year:
2001
Document type:
Article