Determination of photomodified oligodeoxynucleotides by exonuclease digestion, matrix-assisted laser desorption/ionization and post-source decay mass spectrometry.
J Am Soc Mass Spectrom
; 12(10): 1127-35, 2001 Oct.
Article
in En
| MEDLINE
| ID: mdl-11605975
ABSTRACT
A fast method to detect and sequence photomodified oligodeoxynucleotides (ODNs) by exonuclease digestion and matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) is reported. Upon treatment of modified ODNs with both phosphodiesterase I and phosphodiesterase II, the digestion stops at the sites of photomodification. Post-source decay (PSD) of MALDI-produced ions from two enzymatic digestion end products distinguishes isomers such as 5'-d(T[cis-syn]TAAGC) and 5'-d(CGAAT[cis-syn]T), which have symmetrical or identical compositions at the 3' and 5' ends, respectively. Studies have also been done to follow the kinetics for enzyme degradation of photomodified ODNs. The calculated rate constants from a mathematical treatment of the time-dependent MALDI data clearly show that the enzymatic digestion rate slows as the enzyme approaches the modified site.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Oligonucleotides
/
Exonucleases
Language:
En
Journal:
J Am Soc Mass Spectrom
Year:
2001
Document type:
Article
Affiliation country:
Estados Unidos