Crystallization and preliminary X-ray analysis of ocr, the product of gene 0.3 of bacteriophage T7.
Acta Crystallogr D Biol Crystallogr
; 57(Pt 11): 1652-4, 2001 Nov.
Article
in En
| MEDLINE
| ID: mdl-11679734
ABSTRACT
Ocr, the product of gene 0.3 of bacteriophage T7, prevents the action of restriction endonucleases of the host bacteria. The amino-acid sequence of ocr has less than 20% similarity to any protein of known three-dimensional structure. Ocr has been crystallized in a number of different crystal forms and X-ray data for the seleno-L-methionine-substituted form has been collected to a resolution of 1.8 A. The presence of caesium was found to be required for good crystal growth. Anomalous X-ray data was used to identify possible positions for Se and Cs atoms in the unit cell.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Viral Proteins
/
Bacteriophage T7
Language:
En
Journal:
Acta Crystallogr D Biol Crystallogr
Year:
2001
Document type:
Article
Affiliation country:
Reino Unido