An archaeal protein homologous to mammalian SRP54 and bacterial Ffh recognizes a highly conserved region of SRP RNA.
FEBS Lett
; 507(3): 336-40, 2001 Nov 02.
Article
in En
| MEDLINE
| ID: mdl-11696367
ABSTRACT
The gene encoding the 54 kDa protein of signal recognition particle (SRP54) in the hyperthermophilic archaeon Pyrococcus furiosus has been cloned and sequenced. Recombinant P. furiosus SRP54 (pf-SRP54) and the N-terminal G-domain and C-terminal M-domain (pf-SRP54M) of pf-SRP54 with an amino-terminal addition of six histidine residues were expressed in Escherichia coli and subjected to binding experiments for SRP RNA, non-conserved 213-nucleotide RNA (helices 1, 2, 3, 4 and 5) and conserved 107-nucleotide RNA (helices 6 and 8) from SRP RNA. The RNA binding properties of the purified protein were determined by filter binding assays. The histidine-tagged pf-SRP54M bound specifically to the conserved 107-nucleotide RNA in the absence of pf-SRP19, unlike the eukaryotic homologue, with an apparent binding constant (K) of 18 nM.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Signal Recognition Particle
/
Archaeal Proteins
/
Pyrococcus furiosus
/
Escherichia coli Proteins
/
Saccharomyces cerevisiae Proteins
Limits:
Animals
/
Humans
Language:
En
Journal:
FEBS Lett
Year:
2001
Document type:
Article
Affiliation country:
Japón