Effect of N-acetyl-D-glucosamine, and glycerol concentration and equilibration time on acrosome morphology and motility of frozen-thawed boar sperm.
Anim Reprod Sci
; 69(1-2): 91-7, 2002 Jan 23.
Article
in En
| MEDLINE
| ID: mdl-11755720
ABSTRACT
A series of experiments were conducted to determine the effect of N-acetyl-D-glucosamine, glycerol concentration and equilibration time for the freezing of boar spermatozoa in 5 ml maxi-straws. The optimum final glycerol concentration in the diluent with 0.05% N-acetyl-D-glucosamine in the first diluent was 2-3% and the optimum glycerol equilibration time was 2-3h. In conclusion, we recommend the first diluent containing 11% lactose hydrate, 20% egg yolk and 0.05% N-acetyl-D-glucosamine in 100ml distilled water, and the second diluent containing 11% lactose hydrate, 20% egg yolk, 4% glycerol and 1% orvus es paste for the diluents of boar sperm freezing. Also, we found out that 0.05% soluble N-acetyl-D-glucosamine was the optimum concentration in the first diluent and a concentration of 0.05% soluble N-acetyl-D-glucosamine significantly enhanced the cryopreservation of boar spermatozoa.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Acetylglucosamine
/
Semen Preservation
/
Spermatozoa
/
Cryoprotective Agents
/
Glycerol
Limits:
Animals
Language:
En
Journal:
Anim Reprod Sci
Year:
2002
Document type:
Article
Affiliation country:
Corea del Sur