Characterization of stage progression in chronic myeloid leukemia by DNA microarray with purified hematopoietic stem cells.
Oncogene
; 20(57): 8249-57, 2001 Dec 13.
Article
in En
| MEDLINE
| ID: mdl-11781839
ABSTRACT
Chronic myeloid leukemia (CML) is characterized by the clonal expansion of hematopoietic stem cells (HSCs). Without effective treatment, individuals in the indolent, chronic phase (CP) of CML undergo blast crisis (BC), the prognosis for which is poor. It is therefore important to clarify the mechanism underlying stage progression in CML. DNA microarray is a versatile tool for such a purpose. However, simple comparison of bone marrow mononuclear cells from individuals at different disease stages is likely to result in the identification of pseudo-positive genes whose change in expression only reflects the different proportions of leukemic blasts in bone marrow. We have therefore compared with DNA microarray the expression profiles of 3456 genes in the purified HSC-like fractions that had been isolated from 13 CML patients and healthy volunteers. Interestingly, expression of the gene for PIASy, a potential inhibitor of STAT (signal transducer and activator of transcription) proteins, was down-regulated in association with stage progression in CML. Furthermore, forced expression of PIASy has induced apoptosis in a CML cell line. These data suggest that microarray analysis with background-matched samples is an efficient approach to identify molecular events underlying the stage progression in CML.
Search on Google
Collection:
01-internacional
Database:
MEDLINE
Main subject:
RNA, Neoplasm
/
Hematopoietic Stem Cells
/
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
/
Oligonucleotide Array Sequence Analysis
/
Gene Expression Profiling
/
Intracellular Signaling Peptides and Proteins
Type of study:
Diagnostic_studies
/
Evaluation_studies
/
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
Oncogene
Journal subject:
BIOLOGIA MOLECULAR
/
NEOPLASIAS
Year:
2001
Document type:
Article
Affiliation country:
Japón