Clinical and immunological efficacy of intradermal vaccine plus lamivudine with or without interleukin-2 in patients with chronic hepatitis B.
J Med Virol
; 66(4): 452-60, 2002 Apr.
Article
in En
| MEDLINE
| ID: mdl-11857521
ABSTRACT
To evaluate therapeutic immunostimulation nine chronic hepatitis B patients received six monthly intradermal vaccinations with HBsAg in combination with daily lamivudine. Another five patients received six doses of the vaccine and daily lamivudine together with daily Interleukin-2 (IL-2) s.c. within 3 months in an open-labeled trial. Clinical efficacy was assessed by alanine transaminase levels and HBV serology. The induction of specific T and B cell responses was analyzed serially by 3H-thymidine uptake, ELISA and ELISPOT assays. After the therapy was stopped, seven of nine vaccine/lamivudine and two of five vaccine/lamivudine/IL-2 recipients did not have detectable HBV DNA. Four complete responders cleared the virus and had normalized ALT levels, however, one of these patients showed transient disease reactivation followed by spontaneous viral clearance and normal ALT five months later. Low frequencies of anti-HBs producing B cells and HBV specific T helper cells secreting predominantly interferon-gamma were induced by i.d. vaccine therapy. The ELISPOT technique demonstrated transient induction of HBV peptide specific cytotoxic T cells in seven HLA-A2 positive chronic HBV carriers. The preliminary data from this study demonstrate that the HBV surface antigen vaccine in combination with antiviral or immunomodulating drugs induced antiviral immune responses and consequently viral elimination may be achieved in patients with unfavorable prognosis.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Antiviral Agents
/
Vaccines, Synthetic
/
Interleukin-2
/
Hepatitis B Vaccines
/
Reverse Transcriptase Inhibitors
/
Lamivudine
/
Hepatitis B, Chronic
Type of study:
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
J Med Virol
Year:
2002
Document type:
Article
Affiliation country:
Alemania