CD95/CD95 ligand-independent potentiation of treosulfan cytotoxicity by BSO in malignant glioma cells in vitro and in vivo.
Int J Oncol
; 21(1): 213-20, 2002 Jul.
Article
in En
| MEDLINE
| ID: mdl-12063571
ABSTRACT
The glutathione synthase inhibitor, buthionine sulfoximine (BSO), specifically enhances the cytotoxic effects of treosulfan in human glioma cells. BSO depletes glutathione and greatly enhances treosulfan cytotoxicity in all the 12 human malignant glioma cell lines examined. None of these cell lines showed enhanced susceptibility to CD95L- or tumor necrosis factor (TNF)-alpha-induced apoptosis when glutathione is depleted. The combination of serial systemic BSO applications (300 mg/kg) and a single systemic dose of treosulfan (2.5 g/kg) reduced the growth of intracranially growing rat C6 gliomas in vivo by 73% whereas treosulfan alone reduced tumor growth by 16% and BSO alone had no effect. BSO lowered glutathione levels to 25-30% in peripheral blood mononuclear cells (PBMC) and to 50% in the glioma tissue. The glutathione levels in the non-tumor-bearing contralateral hemisphere were unaffected by systemic BSO treatment. The main side effects of treosulfan, gastrointestinal and bone marrow toxicity, were not significantly enhanced by BSO.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Brain Neoplasms
/
Busulfan
/
Membrane Glycoproteins
/
Antineoplastic Agents, Alkylating
/
Fas Receptor
/
Buthionine Sulfoximine
/
Glioma
/
Antimetabolites, Antineoplastic
Limits:
Animals
/
Humans
Language:
En
Journal:
Int J Oncol
Journal subject:
NEOPLASIAS
Year:
2002
Document type:
Article
Affiliation country:
Alemania