C-terminal truncation of WT1 delays but does not abolish hematopoiesis in embryoid bodies.
Blood Cells Mol Dis
; 28(3): 428-35, 2002.
Article
in En
| MEDLINE
| ID: mdl-12367587
ABSTRACT
The effect of mutations that truncate the WT1 protein on in vitro hematopoietic differentiation from embryonal stem cells has been examined by CFU-A assay, o-dianisidine staining for heme, and RT-PCR analysis of the expression of fetal and adult globins. In two independently isolated ES cell lines the mutations delay but do not abolish hematopoiesis. Analysis of replated CFU-A colonies indicates that the delay occurs prior to the formation of hematopoietic stem cells. The results demonstrate a role for WT1 at the onset of hematopoiesis.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Stem Cells
/
WT1 Proteins
/
Embryo, Mammalian
/
Hematopoiesis
/
Mutation
Limits:
Animals
Language:
En
Journal:
Blood Cells Mol Dis
Journal subject:
HEMATOLOGIA
Year:
2002
Document type:
Article
Affiliation country:
Reino Unido