Prediction of fetal D status from maternal plasma: introduction of a new noninvasive fetal RHD genotyping service.
Transfusion
; 42(8): 1079-85, 2002 Aug.
Article
in En
| MEDLINE
| ID: mdl-12385421
ABSTRACT
BACKGROUND:
Invasive procedures to obtain fetal DNA for prenatal blood grouping present a risk to the fetus. During pregnancy, cell-free fetal DNA is present in maternal blood. The detection of RHD sequences in maternal plasma has been used to predict fetal D status, based on the assumption that RHD is absent in D- genomes. STUDY DESIGN ANDMETHODS:
Real-time PCR assays were designed to distinguish RHD from RHDpsi (possessed by the majority of D- black Africans). Plasma-derived DNA from 137 D- women was subjected to real-time PCR to detect fetal RHD and Y chromosome-associated SRY sequences. The accuracy of RHD genotyping from maternal plasma was investigated by comparing results with those obtained by conventional RHD genotyping from fetal tissue or serologic tests on the infant's RBCs. The quantity of fetal DNA in maternal plasma was investigated in 94 pregnancies.RESULTS:
Fetal D status was predicted with 100-percent accuracy from maternal plasma. The number of copies of fetal DNA in maternal plasma was found to increase with gestation.CONCLUSION:
Combination of the sensitivity of real-time PCR with an improved RHD typing assay to distinguish RHD from RHDpsi enables highly accurate prediction of fetal D status from maternal plasma. This has resulted in the implementation of a clinical noninvasive fetal RHD genotyping service.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Pregnancy
/
Rho(D) Immune Globulin
/
Fetal Blood
Type of study:
Diagnostic_studies
/
Prognostic_studies
/
Risk_factors_studies
Limits:
Female
/
Humans
/
Male
Language:
En
Journal:
Transfusion
Year:
2002
Document type:
Article
Affiliation country:
Reino Unido