Isolation of GIF from porcine brain and studies of its zinc transfer kinetics with apo-carbonic anhydrase.
Biometals
; 15(4): 421-7, 2002 Dec.
Article
in En
| MEDLINE
| ID: mdl-12405537
Neuronal growth inhibitory factor (GIF) of porcine brain, was isolated and purified by a similar procedure which was used on the isolation of human and bovine GIF. The native porcine protein with stoichiometry of 4Cu+, 3Zn2+ was obtained for the first time. The kinetics of zinc transfer from Cu4Zn3MT-3 to apo-carbonic anhydrase were studied, and zinc transfer rate constants and thermodynamic parameters were obtained. It is found that like other MTs, porcine Cu4Zn3MT-3 can also transfer its zinc atom to apoCA, even much easier than other MTs. A possible association mechanism has been proposed, the formation of Cu4Zn3MT3-apoCA complex may be the rate-determining step. The obtained data indicate besides its neuronal growth inhibitory function, GIF might play a role in cellular Zn homeostasis in brain.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Swine
/
Zinc
/
Carbonic Anhydrases
/
Nerve Tissue Proteins
Limits:
Animals
Language:
En
Journal:
Biometals
Journal subject:
BIOQUIMICA
Year:
2002
Document type:
Article
Affiliation country:
China
Country of publication:
Países Bajos