[The study of a HPRT locus-specific transgenic method based on FLP recombinase mediated cassette exchange].
Shi Yan Sheng Wu Xue Bao
; 34(4): 279-82, 2001 Dec.
Article
in Zh
| MEDLINE
| ID: mdl-12549206
ABSTRACT
Exchange plasmid pF-HPRT-F3 and Flp expression plasmid pCMV-Flp were constructed and then introduced using electroporation system into F18 ES cell line, which have an exchange cassette F-Neo-F3 at HPRT locus. After HAT selection, HAT resistant clones were obtained. Then G418 sensitivity test and Southern blotting were carried out to screen RMCE recombinants. The results indicated that RMCE had taken place in three of 12 HAT resistant clones. The frequency is 25%. The result demonstrates that it is realizable to introduce transgene to HPRT locus by using Flp recombinase mediated cassette exchange reaction.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
DNA Nucleotidyltransferases
/
Hypoxanthine Phosphoribosyltransferase
Limits:
Animals
Language:
Zh
Journal:
Shi Yan Sheng Wu Xue Bao
Year:
2001
Document type:
Article
Affiliation country:
China