[The PTD domain of Tat protein enhance GFP protein delivering into myeloma cell SP2/0].
Sheng Wu Gong Cheng Xue Bao
; 18(5): 644-7, 2002 Sep.
Article
in Zh
| MEDLINE
| ID: mdl-12561218
ABSTRACT
In order to detect the protein delivery mediated by the PTD (protein transduction domain) of TAT Protein, a expression vector, named pT7460-GFP, was constructed by insert the PTD DNA Sequence, followed by a GFP (green fluorescent protein) gene fused in-frame, into the pT7450 vector. The TAT-GFP fusion protein was expressed in the E. coli ER2566. Most of the fusion protein was presented in the inclusion body. The protein was purified by Ni2+ affinity chromatography under denature conditions, then by a Sepharose Q column to remove urea. The soluble denatured protein was added directly to medium containing the Myeloma Cell SP2/0. It came out that the fusion protein could be detected delivered into the cells under fluorescent microscope in a short time.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Recombinant Fusion Proteins
/
Gene Products, tat
/
Luminescent Proteins
/
Multiple Myeloma
Limits:
Humans
Language:
Zh
Journal:
Sheng Wu Gong Cheng Xue Bao
Journal subject:
BIOTECNOLOGIA
Year:
2002
Document type:
Article
Affiliation country:
China