[Study on the technique of Rehmannia glutinosa virus-free].

ABSTRACT

The stem tip from germ-free stem segment of Rehmannia glutinosa cultured in test tube can be induced into virus-free seedling. The experiment showed that the proper disinfectant for stem segments of Rehmannia glutinosa was 0.05% HgCl2. The seedings from stem segments grew better with MS in the concentration of agar 0.7% and pH7. The stem tips could be directly induced to seedlings by using MS + 6-BA(0.05 mg/L). The MS media for seedlings virus-free culture are 1/4 macro-elements + 1/2 micro-elements and using edible sugar instead of sucrose, so the cost of media could be decreased to 48.7%.