Perils of immunohistochemistry: variability in staining specificity of commercially available COX-2 antibodies on human colon tissue.
Dig Dis Sci
; 48(1): 197-202, 2003 Jan.
Article
in En
| MEDLINE
| ID: mdl-12645811
ABSTRACT
We assessed the immunohistochemical (IHC) staining patterns of three commercially available COX-2 antibodies in human tissues. The location of positive stain in sequential serial 4-mu sections of formalin fixed, paraffin embedded tissue differed considerably. Staining patterns ranged from diffuse cytoplasmic to occasional perinuclear staining. Thus, marked variability in staining results from use of different antibodies, making it important to consider the antibody used when comparing reports of COX-2 staining from different investigators.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Peroxidases
/
Prostaglandin-Endoperoxide Synthases
/
Colon
/
Isoenzymes
Limits:
Humans
Language:
En
Journal:
Dig Dis Sci
Year:
2003
Document type:
Article
Affiliation country:
Estados Unidos