Methods for the exclusion of circoviruses and gammaherpesviruses from pigs.
Xenotransplantation
; 10(4): 343-8, 2003 Jul.
Article
in En
| MEDLINE
| ID: mdl-12795683
ABSTRACT
The use of porcine tissues is being developed as a means to alleviate the shortage of allogeneic tissues and organs available for transplantation. To reduce the possibility of a microorganism of pigs being inadvertently transferred to the recipient of the xenograft, recommendations have been published on the microbiological specifications for organ source pigs. The porcine circoviruses (PCV1 and PCV2) and porcine lymphotropic herpesviruses (PLHV1 and PLHV2) are two infectious agents of pigs which are considered to be of significance for the microbiological safety of xenotransplantation. To ensure the exclusion of these microorganisms from animals destined for use under clinical conditions, reliable breeding methodologies are required. We investigated the efficiency of established derivation procedures for the removal of PCV and PLHV. In comparison with conventionally reared pigs, caesarian and barrier derived animals showed a markedly reduced prevalence of PCVs and PLHVs. Our results indicate that the derivation of animals free of both of these microorganisms is achievable and will enhance the microbiological safety of xenotransplantation.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Transplantation, Heterologous
/
Gammaherpesvirinae
/
Circovirus
/
Circoviridae Infections
/
Herpesviridae Infections
Type of study:
Guideline
/
Prevalence_studies
/
Risk_factors_studies
Limits:
Animals
/
Pregnancy
Language:
En
Journal:
Xenotransplantation
Journal subject:
TRANSPLANTE
Year:
2003
Document type:
Article
Affiliation country:
Reino Unido