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Cloning and expression of alpha-D-glucosidase and N-acetyl-beta-glucosaminidase from the periodontal pathogen, Tannerella forsythensis (Bacteroides forsythus).
Hughes, C V; Malki, G; Loo, C Y; Tanner, A C R; Ganeshkumar, N.
Affiliation
  • Hughes CV; Department of Pediatric Dentistry, Goldman School of Dental Medicine, Boston University, Boston, MA 02118, USA.
Oral Microbiol Immunol ; 18(5): 309-12, 2003 Oct.
Article in En | MEDLINE | ID: mdl-12930523
ABSTRACT
Screening a genomic library of Tannerella forsythensis (Bacteroides forsythus), using synthetic substrates conjugated to a fluorogen, 4-methylumbelliferone identified two glycosidase genes, which encode alpha-D-glucosidase and N-acetyl-beta-D-glucosaminidase, respectively. The alpha-D-glucosidase has a Mr of 81,141 and is homologous to an alpha-D-glucosidase from Bacteroides thetaiotaomicron. The N-acetyl-beta-D-glucosaminidase has a Mr of 87,787 and is homologous to an N-acetyl-beta-D-glucosaminidase in Porphyromonas gingivalis W83.
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Collection: 01-internacional Database: MEDLINE Main subject: Acetylglucosaminidase / Bacterial Proteins / Bacteroides / Alpha-Glucosidases Limits: Humans Language: En Journal: Oral Microbiol Immunol Journal subject: ALERGIA E IMUNOLOGIA / MICROBIOLOGIA / ODONTOLOGIA Year: 2003 Document type: Article Affiliation country: Estados Unidos
Search on Google
Add to My VHL
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PubMed Links

Collection: 01-internacional Database: MEDLINE Main subject: Acetylglucosaminidase / Bacterial Proteins / Bacteroides / Alpha-Glucosidases Limits: Humans Language: En Journal: Oral Microbiol Immunol Journal subject: ALERGIA E IMUNOLOGIA / MICROBIOLOGIA / ODONTOLOGIA Year: 2003 Document type: Article Affiliation country: Estados Unidos