Molecular analysis of an esterase-encoding gene from a lipolytic psychrotrophic pseudomonad.
J Gen Microbiol
; 138(4): 701-8, 1992 Apr.
Article
in En
| MEDLINE
| ID: mdl-1339464
ABSTRACT
An esterase gene (estA) from a lipolytic psychotroph (Pseudomonas sp. LS107d2), was cloned in Escherichia coli and its nucleotide sequence was determined, revealing an ORF encoding a polypeptide of 389 amino acid residues, with a molecular mass of 42276 Da. Labelling of plasmid-encoded proteins with [35S]methionine, using the maxicell procedure, gave a single polypeptide of molecular mass 42 kDa, consistent with that calculated from the ORF. Colonies of E. coli cells containing estA produced a clear halo when grown on solid media containing tributyrin; no clearance was produced when cells were grown on media containing triolein. Extracts of cells containing estA also hydrolysed water-soluble nitrophenol esters, but were unable to cleave water-insoluble substrates. The preference for water-soluble substrates indicates that the gene product is an esterase.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Pseudomonas
/
Esterases
/
Genes, Bacterial
/
Lipolysis
Limits:
Animals
Language:
En
Journal:
J Gen Microbiol
Year:
1992
Document type:
Article
Affiliation country:
Australia