A practical test for in vitro evaluation of photosensitization: assessment with hematoporphyrin derivative (HPD).
Cytotechnology
; 3(2): 171-8, 1990 Mar.
Article
in En
| MEDLINE
| ID: mdl-1368556
ABSTRACT
A simple procedure is described for the determination of the photosensitizing potency of drugs, using three leukemic cell lines, two of lymphocytic origin, L1210 and P388 and one of erythroid type, Friend-745. The procedure allows one to investigate several aspects of the photosensitization properties of tested compounds such as cellular localization and direct (trypan blue exclusion) or delayed (clonogenicity) photomediated toxicities. The method was assessed using crude hematoporphyrin derivative (HPD) as well as dihematoporphyrin ether (DHE) or commercially available Photofrin II. Results were compared to those obtained with normal cells, e.g spleen lymphocytes and erythropoietic stem cells (CFU-e), and discussed in the light of the relative response of normal versus transformed cells.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Hematoporphyrin Photoradiation
/
Tumor Cells, Cultured
/
Hematoporphyrins
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Cytotechnology
Journal subject:
BIOTECNOLOGIA
/
GENETICA
Year:
1990
Document type:
Article
Affiliation country:
Francia