The promoter of the human cystic fibrosis transmembrane conductance regulator gene directing SV40 T antigen expression induces malignant proliferation of ependymal cells in transgenic mice.
Oncogene
; 7(5): 993-7, 1992 May.
Article
in En
| MEDLINE
| ID: mdl-1373882
ABSTRACT
Transgenic mice bearing a human cystic fibrosis transmembrane conductance regulator (CFTR) promoter-SV40 T antigen fusion transgene were generated in order to localize in vivo the potential oncogenesis linked to the tissue-specific activity of the promoter for the CFTR gene. Surprisingly, the only site of tumors resulting from expression of the reporter onc gene was ependymal cells lining the brain ventricles. SV40 T antigen expression in these cells led to a consistent pathology in the first weeks of age ependymoma and consequent hydrocephaly. Tumor-derived cell lines were established, characterized and shown to originate from SV40 T antigen-induced ependymoma. No pathological alterations were found in other organs, such as lungs and pancreas, in which cystic fibrosis is pathologically manifest in humans. Such transgenic mice and derived cell lines may represent valid models for analysing (1) the role of SV40 T antigen in ependymoma formation and (2) CFTR function in ependymal cells.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Gene Expression Regulation, Viral
/
Cell Transformation, Neoplastic
/
Genes, Regulator
/
Promoter Regions, Genetic
/
Simian virus 40
/
Ependyma
/
Membrane Proteins
/
Antigens, Viral, Tumor
Type of study:
Etiology_studies
Limits:
Animals
Language:
En
Journal:
Oncogene
Journal subject:
BIOLOGIA MOLECULAR
/
NEOPLASIAS
Year:
1992
Document type:
Article
Affiliation country:
Francia