X-ray laser microscopy of rat sperm nuclei.
Science
; 258(5080): 269-71, 1992 Oct 09.
Article
in En
| MEDLINE
| ID: mdl-1411525
ABSTRACT
The development of high brightness and short pulse width (< 200 picoseconds) x-ray lasers now offers biologists the possibility of high-resolution imaging of specimens in an aqueous environment without the blurring effects associated with natural motions and chemical erosion. As a step toward developing the capabilities of this type of x-ray microscopy, a tantalum x-ray laser at 44.83 angstrom wavelength was used together with an x-ray zone plate lens to image both unlabeled and selectively gold-labeled dried rat sperm nuclei. The observed images show approximately 500 angstrom features, illustrate the importance of x-ray microscopy in determining chemical composition, and provide information about the uniformity of sperm chromatin organization and the extent of sperm chromatin hydration.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Spermatozoa
/
Cell Nucleus
/
Lasers
/
Microscopy
Limits:
Animals
Language:
En
Journal:
Science
Year:
1992
Document type:
Article