Molecular features of non-B, non-C hepatocellular carcinoma: a PCR-array gene expression profiling study.
J Hepatol
; 39(6): 1004-12, 2003 Dec.
Article
in En
| MEDLINE
| ID: mdl-14642619
ABSTRACT
BACKGROUND/AIMS:
Hepatocellular carcinoma (HCC) usually develops following chronic liver inflammation caused by hepatitis C or B virus. Through expression profiling in a rare type of HCC, for which the causes are unknown, we sought to find key genes responsible for each step of hepatocarcinogenesis in the absence of viral influence.METHODS:
We used 68 non-B, non-C liver tissues (20 HCC, 17 non-tumor, 31 normal liver) for expression profiling with PCR-array carrying 3072 genes known to be expressed in liver tissues. To select the differentially expressed genes, we performed random permutation testing. A weighted voting classification algorithm was used to confirm the reliability of gene selection. We then compared these genes with the results of previous expression profiling studies.RESULTS:
A total of 220 differentially expressed genes were selected by random permutation tests. The classification accuracies using these genes were 91.8, 92.0 and 100.0% by a leave-one-out cross-validation, an additional PCR-array dataset and a Stanford DNA microarray dataset, respectively. By comparing our results with previous reports on virus-infected HCC, four genes (ALB, A2M, ECHS1 and IGFBP3) were commonly selected in some studies.CONCLUSIONS:
The 220 differentially expressed genes selected by PCR-array are potentially responsible for hepatocarcinogenesis in the absence of viral influence.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Hepatitis E
/
Carcinoma, Hepatocellular
/
Gene Expression Profiling
/
Liver Neoplasms
Limits:
Aged
/
Aged80
/
Female
/
Humans
/
Male
/
Middle aged
Language:
En
Journal:
J Hepatol
Journal subject:
GASTROENTEROLOGIA
Year:
2003
Document type:
Article
Affiliation country:
Japón