TRAIL and KILLER are expressed and induce apoptosis in the murine preimplantation embryo.
Biol Reprod
; 71(3): 871-7, 2004 Sep.
Article
in En
| MEDLINE
| ID: mdl-15128592
TRAIL (tumor necrosis factor [TNF]-related apoptosis-inducing ligand) and KILLER are a death-inducing ligand and receptor pair that belong to the TNF and TNF-receptor superfamilies, respectively. To date, only one apoptosis-inducing TRAIL receptor (murine KILLER [MK]) has been identified in mice, and it is a homologue of human Death Receptor 5. Whereas the expression of other death receptors, such as Fas and TNF receptor 1 have been documented in mammalian preimplantation embryos, no evidence currently demonstrates either the presence or the function of TRAIL and its corresponding death receptor, MK. Using reverse transcription-polymerase chain reaction and confocal immunofluorescent microscopy, we found that both TRAIL and MK are expressed from the 1-cell through the blastocyst stage of murine preimplantation embryo development. These proteins are localized mainly at the cell surface from the 1-cell through the morula stage. At the blastocyst stage, both TRAIL and MK exhibit an apical staining pattern in the trophectoderm cells. Finally, using the TUNEL assay, we demonstrated that MK induces apoptosis in blastocysts sensitized to TRAIL via actinomycin D. Taken together, these data are the first to demonstrate the presence and function of TRAIL and MK, a death-inducing ligand and its receptor, in mammalian preimplantation embryos.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Blastocyst
/
Membrane Glycoproteins
/
Tumor Necrosis Factor-alpha
/
Apoptosis
/
Receptors, Tumor Necrosis Factor
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Biol Reprod
Year:
2004
Document type:
Article
Affiliation country:
Estados Unidos
Country of publication:
Estados Unidos