Nuclear localization of the DOCK180/ELMO complex.
Arch Biochem Biophys
; 429(1): 23-9, 2004 Sep 01.
Article
in En
| MEDLINE
| ID: mdl-15288806
ABSTRACT
DOCK180 protein plays a key role during development, cell motility, and phagocytosis. It forms a complex with another protein ELMO, and this complex acts as a guanine nucleotide exchange factor (GEF) for Rac. However, DOCK180-containing complexes have not been purified by unbiased biochemical approaches, and the nature and subcellular localization of these complexes remain unclear. Here, we show that a large fraction of endogenous DOCK180 is present as a 700kDa nuclear complex with ELMO proteins. In addition, this nuclear DOCK180/ELMO complex has functional Rac-GEF activity. Furthermore, endogenous DOCK180 could be found in complexes with different ELMO isoforms (ELMO1, 2 or 3) in different cell lines, depending on the ELMO isoforms expressed. These studies suggest that DOCK180 may associate with different ELMO proteins to form cell-type specific complexes and may have functions in both the nucleus and the cytoplasm.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Carrier Proteins
/
Cell Nucleus
/
Rac GTP-Binding Proteins
/
Cytoplasm
/
Cytoskeletal Proteins
/
Caenorhabditis elegans Proteins
/
Adaptor Proteins, Signal Transducing
Limits:
Animals
/
Humans
Language:
En
Journal:
Arch Biochem Biophys
Year:
2004
Document type:
Article
Affiliation country:
Estados Unidos