[Construction of plasmid with green fluorescent protein gene for analysis of yeasts in yeast wastewater treatment].
Huan Jing Ke Xue
; 25(1): 40-4, 2004 Jan.
Article
in Zh
| MEDLINE
| ID: mdl-15330418
ABSTRACT
Green fluorescent protein (GFP) can be utilized in analysis of the characteristics and distribution of a targeted strain in microbial communities. This study is the first step to establish a dynamic yeast monitoring technique in a wastewater treatment system using yeast by constructing a fluorescent yeast containing gfp gene. The gfp gene was inserted into pACT1-URA3, a powerful plasmid for introducing a foreign gene into Candida boidinii, and then transformed into E. coli JM109. The gfp gene was expressed, though not very highly. The results of the electrophoresis and polymerase chain reaction suggested that the newly constructed plasmid containing gfp gene might not exist in free form in the cells, but in some special way such as interaction with the chromosome.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Yeasts
/
Candida albicans
/
Waste Disposal, Fluid
/
Green Fluorescent Proteins
Language:
Zh
Journal:
Huan Jing Ke Xue
Year:
2004
Document type:
Article
Affiliation country:
China