Different role of functional domains of hTR in DNA binding to telomere and telomerase reconstruction.
FEBS Lett
; 579(1): 127-32, 2005 Jan 03.
Article
in En
| MEDLINE
| ID: mdl-15620701
Even if template sequence of hTR played an essential role in telomere binding, a 326 nucleotide fragment of hTR containing template, pseudoknot, and CR4-5 domains is critical for both binding with telomeric DNA and reconstitution of telomerase activity. A functional study with antisense oligonucleotides suggested that targeted disruption of the template region efficiently abrogated both telomeric DNA binding and telomerase activity, whereas disruption of the CR4-5 region induced only loss of telomerase activity. hTR interacts with telomeric DNA via structural region composed of the template, pseudoknot, and CR4-5 domains, however, each structural domain plays a distinct role in telomere binding and telomerase activity reconstitution.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Telomere
/
Telomerase
/
RNA, Untranslated
Limits:
Humans
Language:
En
Journal:
FEBS Lett
Year:
2005
Document type:
Article
Country of publication:
Reino Unido