Mutational analysis of the adcCBA genes in Streptococcus gordonii biofilm formation.
Oral Microbiol Immunol
; 20(2): 122-7, 2005 Apr.
Article
in En
| MEDLINE
| ID: mdl-15720574
ABSTRACT
Streptococcus gordonii, a primary colonizer, is part of the pioneer biofilm consortium that initiates dental plaque development on tooth surfaces. An insertion of Tn917-lac transposon into the adcR gene produced a biofilm-defective phenotype. S. gordonii adcR is a regulatory gene and is part of an operon (adc) that includes three other genes, adcCBA. AdcC contains a putative consensus-binding site for adenosine triphosphate, AdcB is a putative hydrophobic membrane protein, and AdcA is a putative lipoprotein permease. Mutants were constructed by insertional inactivation in each of the three adcCBA genes and their effects on biofilm formation examined. The adcCspec(R) and adcBspec(R) mutations displayed a biofilm-defective phenotype, whereas the adcAspec(R) mutant was biofilm-positive in a static polystyrene microtiter plate biofilm assay. All three mutants formed poor biofilms in a flow-cell system and were competence-defective, suggesting the adc operon plays an important role in S. gordonii biofilm formation and competence.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Streptococcus
/
Bacterial Proteins
/
Bacterial Adhesion
/
Biofilms
/
Genes, Bacterial
Language:
En
Journal:
Oral Microbiol Immunol
Journal subject:
ALERGIA E IMUNOLOGIA
/
MICROBIOLOGIA
/
ODONTOLOGIA
Year:
2005
Document type:
Article
Affiliation country:
Estados Unidos