A new approach to an influenza live vaccine: modification of the cleavage site of hemagglutinin.
Nat Med
; 11(6): 683-9, 2005 Jun.
Article
in En
| MEDLINE
| ID: mdl-15924146
ABSTRACT
A promising approach to reduce the impact of influenza is the use of an attenuated, live virus as a vaccine. Using reverse genetics, we generated a mutant of strain A/WSN/33 with a modified cleavage site within its hemagglutinin, which depends on proteolytic activation by elastase. Unlike the wild-type, which requires trypsin, this mutant is strictly dependent on elastase. Both viruses grow equally well in cell culture. In contrast to the lethal wild-type virus, the mutant is entirely attenuated in mice. At a dose of 10(5) plaque-forming units, it induced complete protection against lethal challenge. This approach allows the conversion of any epidemic strain into a genetically homologous attenuated virus.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Influenza A virus
/
Influenza Vaccines
/
Hemagglutinin Glycoproteins, Influenza Virus
Limits:
Animals
/
Humans
Language:
En
Journal:
Nat Med
Journal subject:
BIOLOGIA MOLECULAR
/
MEDICINA
Year:
2005
Document type:
Article
Affiliation country:
Alemania