In situ generation of Co(II) by use of a solid-phase reactor in an FIA assembly for the spectrophotometric determination of penicillamine.
J Pharm Biomed Anal
; 39(1-2): 281-4, 2005 Sep 01.
Article
in En
| MEDLINE
| ID: mdl-15936165
ABSTRACT
A flow injection analysis (FIA) manifold for the determination of penicillamine in pharmaceutical preparations is proposed. The manifold includes a solid-phase reactor for the in situ production of the derivatizing reagent, Co(II) ion, which forms a coloured complex with penicillamine in an alkaline medium. The reactor is prepared by natural immobilization of cobalt carbonate on a polymer matrix, which endows it with a high mechanical and microbiological stability. The cobalt released by passage of a 5 x 10(-4) mol l(-1) sulphuric acid stream at a flow-rate of 2.3 ml min(-1) is merged with a volume of 314 microl of sample containing penicillamine in ammonium-ammonia buffer at pH 9.5 to measure the absorbance at 360 nm. Beer's law is obeyed over the penicillamine concentration range 5-60 mg l(-1). The limit of detection (LOD) of the method is 1 mg l(-1) and its throughput 70 samples h(-1).
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Penicillamine
/
Spectrum Analysis
/
Flow Injection Analysis
/
Cobalt
Language:
En
Journal:
J Pharm Biomed Anal
Year:
2005
Document type:
Article
Affiliation country:
España