Anti-protein S antibodies following a varicella infection: detection, characterization and influence on thrombin generation.
J Thromb Haemost
; 3(6): 1243-9, 2005 Jun.
Article
in En
| MEDLINE
| ID: mdl-15946215
ABSTRACT
Postinfectious purpura fulminans is a rare disease. Varicella is one of the precipitating conditions and we recently observed such a case. The 4-year-old child was found to have a severe transient protein S deficiency. By enzyme-linked immunosorbent assay and surface plasmon resonance we first demonstrated that anti-protein S antibodies were present and also transient. Next we characterized the epitopes against which these antibodies were directed and found that they predominantly recognized the N-terminal part of protein S. Finally we showed by thrombography a transient dramatic hypercoagulable state as a result of thrombin being unregulated by the dynamic protein C inhibitory system in vitro thrombin generation, in response to a low concentration of tissue factor, was almost insensitive to activated protein C up to 25 nmol L(-1) on day 4 while it was normally sensitive on day 42. For the first time, we demonstrated a temporal relationship between protein S deficiency, antibodies to protein S and hypercoagulability, thus supporting the pathogenic role of these antibodies.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Autoantibodies
/
Thrombin
/
Chickenpox
/
Protein S
Type of study:
Diagnostic_studies
/
Etiology_studies
Limits:
Child, preschool
/
Humans
/
Male
Language:
En
Journal:
J Thromb Haemost
Journal subject:
HEMATOLOGIA
Year:
2005
Document type:
Article
Affiliation country:
Francia