The crystal structure of the Methanocaldococcus jannaschii multifunctional L7Ae RNA-binding protein reveals an induced-fit interaction with the box C/D RNAs.
Biochemistry
; 44(28): 9657-72, 2005 Jul 19.
Article
in En
| MEDLINE
| ID: mdl-16008351
ABSTRACT
Archaeal ribosomal protein L7Ae is a multifunctional RNA-binding protein that recognizes the K-turn motif in ribosomal, box H/ACA, and box C/D sRNAs. The crystal structure of Methanocaldococcus jannaschii L7Ae has been determined to 1.45 A, and L7Ae's amino acid composition, evolutionary conservation, functional characteristics, and structural details have been analyzed. Comparison of the L7Ae structure to those of a number of related proteins with diverse functions has revealed significant structural homology which suggests that this protein fold is an ancient RNA-binding motif. Notably, the free M. jannaschii L7Ae structure is essentially identical to that with RNA bound, suggesting that RNA binding occurs through an induced-fit interaction. Circular dichroism experiments show that box C/D and C'/D' RNA motifs undergo conformational changes when magnesium or the L7Ae protein is added, corroborating the induced-fit model for L7Ae-box C/D RNA interactions.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Ribosomal Proteins
/
RNA-Binding Proteins
/
Methanococcus
/
Ribonucleoproteins, Small Nuclear
/
Archaeal Proteins
/
RNA, Archaeal
Type of study:
Prognostic_studies
Language:
En
Journal:
Biochemistry
Year:
2005
Document type:
Article
Affiliation country:
Estados Unidos