Her-2/neu expression in breast cancer--A comparison of different diagnostic methods.
Anticancer Res
; 25(3B): 1895-900, 2005.
Article
in En
| MEDLINE
| ID: mdl-16158923
ABSTRACT
BACKGROUND:
Determination of Her-2/neu overexpression in breast cancer has previously been shown to be of prognostic significance. In this study, Her-2/neu expression in breast cancer was characterised by real-time PCR (RLT-PCR) based LightCycler-HER-2/neu DNA Quantification with immunohistochemistry (IHC) and fluorescence in situ hybridisation (FISH). MATERIAL ANDMETHODS:
Fifteen specimens of invasive breast cancer - whole tissue sections as well as microdissected tumour cells - were subjected to RLT-PCR. Additionally, IHC and FISH were performed.RESULTS:
Her-2/neu overexpression was detected by FISH and by real-time PCR in the same tumours. In contrast, IHC revealed discordant results.CONCLUSION:
Determination of Her-2/neu amplification by real-time PCR is a sensitive and specific method with some advantages over FISH. This method is simple and reliable and has the potential of categorizing those tumours with borderline Her-2/neu overexpression as determined by IHC.
Search on Google
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Breast Neoplasms
/
Receptor, ErbB-2
Type of study:
Diagnostic_studies
Limits:
Humans
Language:
En
Journal:
Anticancer Res
Year:
2005
Document type:
Article
Affiliation country:
Alemania